After graduating the Tbilisi State University, the faculty of Law, Anano Tsintsabadze started to practice in a law firm, where her main profile was the legal aspects of property restitution. Since then, she has been working as a lawyer in a private sector, focused on the corporate and telecommunications law. Among her activities in the civil society sector, contribution to the environmental legislation development and women's rights protection shall be emphasized.

Currently, Anano is a Social Rights Program Coordinator at Human Rights Monitoring and Education Center and works on the labor rights and issues of homelessness. Additionally, she leads Initiative for a Public Space, nongovernmental organization, that currently, in cooperation with the South Caucasus Regional Office of the Heinrich Boell Foundation, is implementing the project Legal Advocacy For Public Spaces.

Anano Tsintsabadze holds Master of Laws degree from the Chicago Kent College of Law.